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位置: 首页 > 磷酸化抗体 > Her3/ErbB3(phospho-Tyr1328) Antibody

Her3/ErbB3(phospho-Tyr1328) Antibody#11510

Her3/ErbB3(phospho-Tyr1328) Antibody
Her3/ErbB3(phospho-Tyr1328) Antibody
Her3/ErbB3(phospho-Tyr1328) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Her3/ErbB3(phospho-Tyr1328) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Her3/ErbB3 only when phosphorylated at tyrosine 1328.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 1328 (P-D-Y(p)-W-H) derived from Human Her3/ErbB3.

基因/蛋白名称Her3/ErbB3

修饰Phospho

别名 Tyrosine kinase-type cell surface receptor HER3

数据库入口号Swiss-Prot: P21860
NCBI Protein: NP_001005915.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 185kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from A431 cells untreated or treated with EGF using Her3/ErbB3(phospho-Tyr1328) Antibody #11510.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Her3/ErbB3(Phospho-Tyr1328) Antibody #11510(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF7 cells using Her3/ErbB3(phospho-Tyr1328) Antibody #11510.
背景
Binds and is activated by neuregulins and NTAK.

Holbro, T. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 8933-8938.
Kobayashi, M. et al. (2003) Oncogene 22, 1294-1301.
Kim, H.H. et al. (1994) J. Biol. Chem. 269, 24747-24755.
Yarden, Y. and Sliwkowski, M.X. (2001) Nature Rev. Mol. Cell. Biol. 2, 127-137.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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