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位置: 首页 > 磷酸化抗体 > eIF2a(phospho-Ser49) Antibody

eIF2a(phospho-Ser49) Antibody#11511

eIF2a(phospho-Ser49) Antibody
eIF2a(phospho-Ser49) Antibody
eIF2a(phospho-Ser49) Antibody
eIF2a(phospho-Ser49) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称eIF2a(phospho-Ser49) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of eIF2a only when phosphorylated at Serine 49.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 49 (L-L-S(p)-E-L) derived from Human eIF2a.

基因/蛋白名称eIF2a

修饰Phospho

别名Eukaryotic translation initiation factor 2 subunit alpha

数据库入口号Swiss-Prot: P05198
NCBI Protein: NP_004085.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 38kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from Hela cells untreated or treated with starvation using eIF2a(phospho-Ser49) Antibody #11511.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2α (Phospho-Ser49) Antibody #11511 (left) or the same antibody preincubated with blocking peptide #51511 (right).
Immunofluorescence staining of methanol-fixed Hela cells using eIF2a(phospho-Ser49) Antibody #11511.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using eIF2α (Phospho-Ser49) Antibody #11511.
背景

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
De Haro, C. et al. (1996) FASEB J. 10, 1378-1387.
Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128.
Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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