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位置: 首页 > 磷酸化抗体 > ABL1/2 (phospho-Tyr393/439) Antibody

ABL1/2 (phospho-Tyr393/439) Antibody#11530

ABL1/2 (phospho-Tyr393/439) Antibody
ABL1/2 (phospho-Tyr393/439) Antibody
ABL1/2 (phospho-Tyr393/439) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称ABL1/2 (phospho-Tyr393/439) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms

特异性The antibody detects endogenous level of ABL1/2 only when phosphorylated at tyrosine393/439.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 393/439 (D-T-Y(p)-T-A) derived from Human ABL1/2.

基因/蛋白名称ABL1/2

修饰Phospho

别名Abelson murine leukemia viral oncogene homolog 1; c-ABL; p150

数据库入口号Swiss-Prot: P00519 P42684
NCBI Protein: NP_005148.2 NP_001129472.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 210kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/2(Phospho-Tyr393/439) Antibody #11530(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using ABL1/2(phospho-Tyr393/439) Antibody #11530.
Western blot analysis of extracts from HL60 cells using ABL1/2(phospho-Tyr393/439) Antibody #11530 and the same antibody preincubated with blocking peptide.
背景

Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.

Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650.
Danial, N.N. et al. (2000) Oncogene 19, 2523-2531.
Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637.
Pluk, H. et al. (2002) Cell 108, 247-259.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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