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位置: 首页 > 磷酸化抗体 > MARCKS(phospho-Ser170) Antibody

MARCKS(phospho-Ser170) Antibody#11535

MARCKS(phospho-Ser170) Antibody
MARCKS(phospho-Ser170) Antibody
MARCKS(phospho-Ser170) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称MARCKS(phospho-Ser170) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous level of MARCKS only when phosphorylated at threonine 170.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 170 (G-F-S(p)-F-K) derived from Human MARCKS.

基因/蛋白名称MARCKS

修饰Phospho

别名MACS, 80K-L, PKCSL, PRKCSL

数据库入口号Swiss-Prot: P29966
NCBI Protein: NP_002347.5

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 80kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from MCF cells untreated or treated with EGF using MARCKS(phospho-Ser170) Antibody #11535.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using MARCKS(Phospho-Ser170) Antibody #11535(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from 3T3 cells, treated with serum or calf intestinal phosphatase (CIP), using MARCKS (phospho-Ser170) Antibody #11535.
背景

MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Ramsden, J.J. (2000) Int. J. Biochem. Cell Biol. 32, 475-479.
Graff, J. M. et al. (1989) J. Biol. Chem. 264, 21818-21823.
Hartwig, J. H. et al. (1992) Nature 356, 618-622.
Thelen, M. et al. (1991) Nature 351, 320-322.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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