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位置: 首页 > 磷酸化抗体 > c-kit(phospho-Tyr936) Antibody

c-kit(phospho-Tyr936) Antibody#11539

c-kit(phospho-Tyr936) Antibody
c-kit(phospho-Tyr936) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称c-kit(phospho-Tyr936) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC WB

种属反应性Hu

特异性The antibody detects endogenous level of c-kit only when phosphorylated at tyrosine 936.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 936 (H-I-Y(p)-S-N) derived from Human c-Kit.

基因/蛋白名称c-kit

修饰Phospho

别名KIT; CD117; SCFR; PBT;

数据库入口号Swiss-Prot: P10721
NCBI Protein: NP_000213.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 120 145kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from C6 cell untreated or treated with serum usingc-kit(phospho-Tyr936) Antibody #11539.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-kit(Phospho-Tyr936) Antibody #11539(left) or the same antibody preincubated with blocking peptide(right).
背景
This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)

Martin, F.H. et al. (1990) Cell 63, 203-11.
Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.
Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
Nocka, K. et al. (1990) EMBO J 9, 1805-13.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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