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位置: 首页 > 磷酸化抗体 > FAK(phospho-Tyr576/Tyr577) Antibody

FAK(phospho-Tyr576/Tyr577) Antibody#11545

FAK(phospho-Tyr576/Tyr577) Antibody
FAK(phospho-Tyr576/Tyr577) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称FAK(phospho-Tyr576/Tyr577) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of FAK only when phosphorylated at tyrosine 576/577.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 576/tyrosine 577 (S-T-Y(p)-Y(p)-K-A) derived from Human FAK.

基因/蛋白名称FAK

修饰Phospho

别名FADK 1; FAK1; PTK2

数据库入口号Swiss-Prot: Q05397
NCBI Protein: NP_005598.3

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 125kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from HT29 cells untreated or treated with PMA using FAK(phospho-Tyr576/Tyr577) Antibody #11545.
Immunofluorescence staining of methanol-fixed Hela cells using FAK(phospho-Tyr576/Tyr577) Antibody #11545.
背景

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Parsons, J.T. et al. (2000) Oncogene 19, 5606-5613
Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14, 1680-1688.
Cobb, B.S. et al. (1994) Mol. Cell. Biol. 14, 147-155.
Chen, H.C. et al. (1996) J. Biol. Chem. 271, 26329-26334.

如果您使用该产品11545发表了文章,请通知我们,让我们可以引用您的文献。

et al,Cytotoxic activity of Shp2 inhibitor fumosorinone in human cancer cells.In Oncol Lett.On 2018 Jun by Chen C, Xue T et al..PMID:29928374, , (2018),
PMID: 29928374
Jun-shan RUAN, Yu-ping LIU, Lei ZHANG et al,Luteolin reduces the invasive potential of malignant melanoma cells by targeting 娄脗3 integrin and the epithelial-mesenchymal transition., Acta Pharmacologica Sinica., 33: 1325篓C1331 (2012),
PMID: 22983392
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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