产品详情
产品名称STAT1(Ab-701) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC
种属反应性Human Mouse Rat
特异性The antibody detects endogenous level of total STAT1 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa. 699~703 (T-G-Y-I-K) derived from Human STAT1.
基因/蛋白名称STAT1
别名CANDF7, ISGF-3, STAT91
数据库入口号Swiss-Prot: P42224
NCBI Protein: NP _009330.1
实际分子量84,91kd
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 84,91kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
背景
Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120.
Durbin J E, et al. (1996) Cell. 84: 443-450.
Meraz M A, et al. (1996) Cell. 84: 431-442.
Wakao H, et al. (1994) EMBO J. 13: 2182-2191.
Demoulin J, B. et al. (1999) J Biol Chem. 274: 25855-258
Ihle J N, et al. (1994) Trends Biochem Sci. 19: 222-227.
如果您使用该产品21044发表了文章，请通知我们，让我们可以引用您的文献。
et al,Costunolide improved dextran sulfate sodium-induced acute ulcerative colitis in mice through NF-kB, STAT1/3, and Akt signaling pathways. In Int Immunopharmacol on 2020 Jul by Fan Xie, Hai Zhang, et al..PMID:32413737,
, (2020),
PMID: 32413737
et al,A thiazole-derived oridonIn analogue exhibits antitumor activity by directly and allosterically InhibitIng STAT3. In J Biol Chem on 2019 Nov 15;by Shen X, Zhao L,et al..PMID:31594861,
, (2019),
PMID: 31594861
et al,Danzhi Jiangtang Capsule ameliorates kidney injury via inhibition of the JAK-STAT signaling pathway and increased antioxidant capacity in STZ-induced diabetic nephropathy rats.In Biosci Trends.On 2019 Jan 22 by Sun M, Bu W et al..PMID: 30606979 ,
, (2018),
PMID: 30606979
et al,DJC suppresses Advanced Glycation End Products-Induced JAK-STAT signaling and ROS in mesangial cells.In Evid Based Complement Alternat Med on 2017 May 29 by Min Sun, Yan Li ,et al..PMID: 28630633,
, (2017),
PMID: 28630633
et al,Expression of interferon effector gene SART1 correlates with interferon treatment response against hepatitis B infection. In Mediators Inflamm on 2016 by Yong Li, Chuanlong Zhu et al..PMID: 28077916,
, (2016),
PMID: 28077916
et al,ycobacterium tuberculosis EspB protein suppresses interferon-纬-induced autophagy in murine macrophages.In J Microbiol Immunol Infect on 2016 Dec by DanDan Huang, Lang Bao et al..PMID:25641595 ,
, (2016),
PMID: 25641595
et al,IL-17 intensifies IFN-纬-induced NOS2 upregulation in RAW 264.7 cells by further activating STAT1 and NF-魏B.In Int J Mol Med on 2016 byQiyue Gao, Yang Liu et al..PMID:26677135 ,
, (2016),
PMID: 26677135
查看更多
Xiang Cheng, Jing Wang, Ni Xia et al,A guanidine-rich regulatory oligodeoxy-nucleotideimproves type-2 diabetes in obese mice by blocking T-cell differentiation.,
EMBO Mol Med., 4(10):1112篓C1125 (2012),
PMID: 23027613
et al,A Guanidine-Rich Regulatory Oligodeoxynucleotide Improves type-2 Diabetes in Obese Mice by Blocking T-cell Differentiation. In EMBO Mol Med on 2012 Oct by Xiang Cheng, Jing Wang, et al..PMID:23027613
,
, (2012),
PMID: 23027613
有货
