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位置: 首页 > 抗体 > CREB(Ab-133) Antibody

CREB(Ab-133) Antibody#21052

CREB(Ab-133) Antibody
CREB(Ab-133) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称CREB(Ab-133) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC

种属反应性Hu Ms

特异性The antibody detects endogenous level of total CREB protein and it also detects the CREB-related protein ATF-1.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa. 131~135 (R-P-S-Y-R) derived from Human CREB.

基因/蛋白名称CREB

别名CREB-1; CREB1;

数据库入口号Swiss-Prot: P16220
NCBI Protein: NP _004370.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293, HT29 and Hela cells using CREB(Ab-133) Antibody #21052.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB(Ab-133) Antibody #21052(left) or the same antibody preincubated with blocking peptide(right).
背景
This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.

Xing J, et al. (1998) Mol Cell Biol 18(4): 1946-55.
Tan Y, et al.( 1996) EMBO J; 15(17): 4629-42.
Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385.
Mayo LD, et al. (2001) Biol Chem; 276(27): 25184-9.
Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.

如果您使用该产品21052发表了文章,请通知我们,让我们可以引用您的文献。

et al,Connexin Hemichannels Contribute to the Activation of cAMP Signaling Pathway and Renin Production. In Int J Mol Sci on 2020 Jun 23 by Jingru Hong, Jian Yao,et al..PMID:32585970, , (2020),
PMID: 32585970
et al,Aluminum toxicity related to SOD and expression of presenilin and CREB in Bombyx mori.In Arch Insect Biochem Physiol.On 2018 Oct by Liu L, Qian X et al..PMID:29978503, , (2018),
PMID: 29978503
et al,Inhibition of TPL2 by interferon-α suppresses bladder cancer through activation of PDE4D.In J Exp Clin Cancer Res. On 2018 Nov 27 by Qiang Z, Zhou ZY et al..PMID: 30482227, , (2018),
PMID: 30482227
et al,Lithium chloride administration prevents spatial learning and memory impairment in repeated cerebral ischemia-reperfusion mice by depressing apoptosis and increasing BDNF expression in hippocampus.In Behav Brain Res.On 2015 Sep 15 by Fan M, Jin W et al..PMID:26031381 , , (2015),
PMID: 26031381
et al,Hydrogen sulfide decreases 尾-adrenergic agonist-stimulated lung liquid clearance by inhibiting ENaC-mediated transepithelial sodium absorption. In Am J Physiol Regul Integr Comp Physiol on 2015 Apr 1 by Alisa M Agn茅, Jan-Peter Baldin, et al..PMID: 25632025, , (2015),
PMID: 25632025
et al,Downregulation of microsomal glutathione-S-transferase 1 modulates protective mechanisms in differentiated PC12 cells.In J Physiol Biochem on 2014 Jun by Monika Sobczak , Tomasz Boczek et al ..PMID: 24419913, , (2014),
PMID: 24419913
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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