Akt(Ab-473) Antibody - SAB | Signalway Antibody

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Akt(Ab-473) Antibody#21054

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产品名称Akt(Ab-473) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of total Akt protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa. 471~475 (Q-F-S-Y-S) derived from Human Akt.

基因/蛋白名称Akt

别名AKT; C-AKT; PKB; PKB-alpha; RAC

数据库入口号Swiss-Prot: P31749
NCBI Protein: NP_001014431.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from 293 and 3T3 cells using Akt(Ab-473) Antibody #21054.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt(Ab-473) Antibody #21054(left) or the same antibody preincubated with blocking peptide(right).

Western blot analysis of extracts of various cell lines, using Akt(Ab-473) #21054

背景

General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

Baudhuin LM, et al. (2004) FASEB J Feb; 18(2): 341-3.
Min YH, et al. (2004) Cancer Res; 64(15): 5225-31.
Feng J, et al. (2004) J Biol Chem; 279(34): 35510-7.
Ayala G, et al. (2004) Clin Cancer Res; 10(19): 6572-8.
Lungu AO, et al. (2004) J Biol Chem; 279(47): 48794-800.

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et al,Exosomes Derived from Akt -Modified Human Umbilical Cord Mesenchymal Stem Cells Improve Cardiac Regeneration and Promote Angiogenesis via Activating Platelet-Derived Growth Factor D. In Stem Cells Transl Med on 2017 Jan by Jie Ma, Yuanyuan Zhao, et al..PMID:28170176, , (2017),
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应用

WB免疫印迹
IHC免疫组化
IF免疫荧光
ICC免疫细胞化学
FC流式细胞
IP免疫沉淀
E酶联免疫吸附法
DB免疫斑点法
ChIP染色质免疫沉淀
GICA胶体金免疫层析法
NC阴性对照

种属反应性

Hu人
Ms小鼠
Rt大鼠
Dm果蝇
C线虫
Mk猴
Rb兔
B牛
D狗
P猪
Hm仓鼠
ChHm中国仓鼠
Chk鸡

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Akt(Ab-473) Antibody#21054

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Akt(Ab-473) Antibody#21054

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