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位置: 首页 > 抗体 > BCL-2(Ab-56) Antibody

BCL-2(Ab-56) Antibody#21059

BCL-2(Ab-56) Antibody
BCL-2(Ab-56) Antibody
BCL-2(Ab-56) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称BCL-2(Ab-56) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC IF

种属反应性Hu

特异性The antibody detects endogenous level of total BCL-2 protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa. 54~58 (G-H-T-P-H) derived from Human BCL-2.

基因/蛋白名称BCL-2

别名BCL2

数据库入口号Swiss-Prot: P10415
NCBI Protein: NP_000624.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 26kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela, A2780 and 293 cells using BCL-2(Ab-56) Antibody #21059.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-2(Ab-56) Antibody #21059(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using BCL-2(Ab-56) Antibody #21059.
背景
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Ling, Y. H. et al. (1998) J. Biol. Chem. 273, 18984-18991.
Huang, S.J. and Cidlowski, J.A. (2002) FASEB 16, 825-832.
Deng, X. et al. (2001) J. Biol. Chem. 276, 23681-23688.
Huang ST,et al. (2002) FASEB J Jun; 16(8): 825-32.
Yamamoto, K. et al. (1999) Mol. Cell. Biol. 19, 8469-8478.

如果您使用该产品21059发表了文章,请通知我们,让我们可以引用您的文献。

et al,miR-375 inhibits Helicobacter pylori-induced gastric carcinogenesis by blocking JAK2鈥揝TAT3 signaling.In Cancer Immunol Immunother on 2014 Jul by Lin Miao , Kunmei Liu et al..PMID:24718681, , (2014),
PMID: 24718681
Wei-Jun Pang, Yan Xiong, Zhao Zhang et al,Lentivirus-mediated Sirt1 shRNA and resveratrol independently induce porcine preadipocyte apoptosis by canonical apoptotic pathway., Mol Biol Rep., 40(1):129篓C139 (2013),
PMID: 23065251
et al,Anticancer effects of sodium butyrate on hepatocellular carcinoma cells in vitro. In Int J Mol Med on 2013 Apr by Hong-Gang Wang, Xiao-Dan Huang, et al..PMID: 23440283, , (2013),
PMID: 23440283
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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