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位置: 首页 > 抗体 > BCL-2(Ab-70) Antibody

BCL-2(Ab-70) Antibody#21060

BCL-2(Ab-70) Antibody
BCL-2(Ab-70) Antibody
BCL-2(Ab-70) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称BCL-2(Ab-70) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC IF

种属反应性Hu

特异性The antibody detects endogenous level of total BCL-2 protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.68~72 (R-T-S-P-L) derived from Human BCL-2.

基因/蛋白名称BCL-2

别名BCL2

数据库入口号Swiss-Prot: P10415
NCBI Protein: NP_000624.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 26kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from MCF cells using BCL-2(Ab-70) Antibody #21060 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-2(Ab-70) Antibody #21060(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using BCL-2(Ab-70) Antibody #21060.
背景
BCL-2 encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.

Muller IM, et al. (2005) Mol Pharmacol.
Jin Z, Gao F,et al. J Biol Chem 2004 Sep 17; 279(38): 40209-19.
Kumar Biswas S,et al. Mol Cancer Ther 2004 Mar; 3(3): 327-34.
Huang ST,et al. (2002)FASEB J; 16(8): 825-32.
Yamamoto K,et al. (1999) Mol Cell Biol; 19(12): 8469-78.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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