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EGFR Antibody#21073

EGFR Antibody
EGFR Antibody
EGFR Antibody
是否有货: 有货
产品总价:
产品详情

产品名称EGFR Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC IF

种属反应性Hu

特异性The antibody detects endogenous level of total EGFR protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.1068~1072 (R-Y-S-S-D) derived from Human EGFR.

基因/蛋白名称EGFR

别名 Receptor tyrosine-protein kinase ErbB-1; ERBB1;

数据库入口号Swiss-Prot: P00533
NCBI Protein: NP_005219.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 175kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from HUVEC and MDA cells using EGFR(Ab-1070) Antibody #21073.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR(Ab-1070) Antibody #21073(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF cells using EGFR(Ab-1070) Antibody #21073.
背景

Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentia

Feinmesser RL,et al. (1999) J Biol Chem; 274(23): 16168-73.
Gamou S,et al. (1995)FEBS Lett; 357(2): 161-4.
Gamou S, et al. (1994)J Cell Physiol; 158(1): 151-9.
Heisermann GJ, et al. (1988) J Biol Chem; 263(26): 13152-8.

如果您使用该产品21073发表了文章,请通知我们,让我们可以引用您的文献。

Bin Gui, Xiao Han, Yu Zhang et al,Dimerization of ZIP promotes its transcriptional repressive function and biological activity., The International Journal of Biochemistry & Cell Biology, 44 : 886篓C 895 (2012),
PMID: 22387311
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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