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FAK(Ab-861) Antibody#21076

FAK(Ab-861) Antibody
FAK(Ab-861) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称FAK(Ab-861) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous level of total FAK protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa. 859~863 (H-I-Y-Q-P) derived from Human FAK.

基因/蛋白名称FAK

别名FADK 1; FAK1; PTK2

数据库入口号Swiss-Prot: Q05397
NCBI Protein: NP _005598.3

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 125kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HT29, Hela and 293 cells using FAK(Ab-861) Antibody #21076.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using FAK(Ab-861) Antibody #21076(left) or the same antibody preincubated with blocking peptide(right).
背景
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Shi Q, et al. (2003) Mol Biol Cell; 14(10): 4306-15.
Vadlamudi RK, et al. (2003) FEBS Lett; 543(1-3): 76-80.
Eliceiri BP, et al. (2002) J Cell Biol Apr 01; 157(1): 149-60.
Abu-Ghazaleh R, (2001) et al. Biochem J; 360(Pt 1): 255-64.
Slack JK, et al.(2001) Oncogene; 20(10): 1152-63.

如果您使用该产品21076发表了文章,请通知我们,让我们可以引用您的文献。

et al,Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation. In Sci Rep on 2019 Apr 1 by Sonoko Noda, Nobuyuki Kawashima, et al..PMID: 30931957, , (2019),
PMID: 30931957
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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