产品详情
产品名称Annexin V-FITC/PI细胞凋亡检测试剂盒
基因/蛋白名称Annexin V
应用详情
1、 离心收集悬浮细胞。离心机 300-500g，2-8°C，离心时间 5min，弃培养基。（贴壁细胞用不含 EDTA 的胰酶 消化后离心，收集细胞。胰酶消化时间不宜过长，以防引起假阳性）。
2、 用冷 PBS 洗涤细胞两次。（300g，2-8°C，离心时间 5min 收集细胞）。
3、 用 400ul 1X Annexin V 结合液悬浮细胞，浓度大约为 1 X 106 cells/ml。
4、 在细胞悬浮液中加入 5ul Annexin V-FITC 染色液，轻轻混匀后于 2-8°C 避光条件下孵育 15 分钟。
5、 加入 5-10ul PI 染色液后轻轻混匀于 2-8°C 避光条件下孵育 5 分钟。
6、 立即用流式细胞仪或荧光显微镜检测。
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