MTT法细胞增殖及细胞毒性检测试剂盒被广泛应用于细胞增殖和细胞毒性的检测。活细胞的线粒体中存在与NADP相关的脱氢酶，可将黄色的MTT还原生成结晶状的蓝紫色的Formazan，死细胞无此酶，MTT不被还原。在特定溶剂存在的情况下，Formazan可以被完全溶解。然后通过酶标仪可以测定460-630nm波长附近的吸光度。细胞增殖越多越快，则吸光度越高；细胞毒性越大，则吸光度越低。

   本试剂盒灵敏度高，线性范围宽，使用方便。

Introduction : This kit is widely used in cell proliferation and cytotoxic detection. The mitochondria of the live cells have dehydrogenase-related dehydrogen enzymes, which can restore the yellow MTT to generate crystal-shaped blue-purple Formazan. The dead cells have no enzymes and MTT is not restored. Formazan can be fully dissolved in the existence of specific solvents. Then you can measure the absorbance at the wavelength of 460-630nm. The more the cells are proliferated faster, the higher the absorbance; the greater the cytotoxicity, the lower the absorbance. The sensitivity of this kit is high, the linear range is wide, and it is convenient to use.