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位置: 首页 > 抗体 > p53(Ab-46) Antibody

p53(Ab-46) Antibody#21090

p53(Ab-46) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称p53(Ab-46) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous level of total p53 protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa. 44~48 (M-L-S-P-D) derived from Human p53.

基因/蛋白名称p53

别名Tumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13

数据库入口号Swiss-Prot: P04637
NCBI Protein: NP_000537.3

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 53kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HT29 cells using p53(Ab-46) Antibody #21090.
背景
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

Dhavan, R. and Tsai, L.H. (2001) Nat Rev Mol Cell Biol. 2: 749-759.
Patrick, G. N. et al. (1998) J Biol Chem. 273: 24057-24064.
Di Stefano V, et al. (2005) Oncogene. 24(35):5431-5442.
Mayo LD, et al.(2005) J Biol Chem. 280(28):25953-25959.
Wang L, et al. (2005) Oncogene. 24(18): 3020-3027.

如果您使用该产品21090发表了文章,请通知我们,让我们可以引用您的文献。

Lei Zhang, Junshan Ruan, Linggeng Yan et al,Xanthatin Induces Cell Cycle Arrest at G2/M Checkpoint and Apoptosis via Disrupting NF-娄脢B Pathway in A549 Non-Small-Cell Lung Cancer Cells., Molecules., 17:3736-3750 (2012),
PMID: 22450683
et al,Nordihydroguaiaretic acid inhibits growth of cervical cancer SiHa cells by up-regulating p21.In Oncol Lett on 2011 Jan by Peng Gao, Fei Zhai,et al..PMID:22870140, , (2011),
PMID: 22870140
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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