产品详情
产品名称Jak3 Antibody Biotin Conjugated
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Purified by Protein A.
应用WB IHC-P
种属反应性Hu Ms Rt
免疫原描述KLH conjugated synthetic peptide aa 610-660 1124 derived from human Jak3
标记Biotin
激发/发射波长N A
基因/蛋白名称Jak3
别名JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN; Tyrosine-protein kinase JAK3; Janus kinase 3; Leukocyte janus kinase; JAK3
数据库入口号Swiss-Prot#P52333
NCBI Gene ID3718
细胞定位Cytoplasm
浓度1mg ml
配方10mM Tris Buffered Saline containing 1% BSA, 50% glycerol and 0.09% sodium azide.
保存 Store at 4C for 12 months.
应用详情
Western blotting: 1:100-1000
Immunohistochemistry1:100-500
背景
Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.