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IKK a(Ab-23) Antibody#21123

IKK a(Ab-23) Antibody
IKK a(Ab-23) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称IKK a(Ab-23) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of total IKKa protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.21~25 (L-G-T-G-G) derived from Human IKK a.

基因/蛋白名称IKK a

别名I kappa-B kinase alpha; I-kappa-B kinase 1; IKK-A; IKK-alpha; IKK1

数据库入口号Swiss-Prot: O15111
NCBI Protein: NP_001269.3

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 85kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela, 293 and 3T3 cells using IKK a(Ab-23) Antibody #21123.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IKK a(Ab-23) Antibody #21123(left) or the same antibody preincubated with blocking peptide(right).
背景
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.

Yuan ZQ, et al.(2002)J Biol Chem; 277(33): 29973-82.
Ozes ON, et al. (1999)Nature; 401(6748): 82-5.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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