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AMPKα1(Ab-496) Antibody#21130

AMPKα1(Ab-496) Antibody
AMPKα1(Ab-496) Antibody
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产品总价:
产品详情

产品名称AMPKα1(Ab-496) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous level of total AMPKα1 protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.494~498 (S-G-S-V-S) derived from Human AMPKα1.

基因/蛋白名称AMPKα1

别名 AAPK1; AMPK alpha-1 chain; AMPK-alpha1; HMG-CoA redustase kinase; PRKAA1

数据库入口号Swiss-Prot: Q13131/P54646
NCBI Protein: NP_006242.5

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 63kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from 293 cells using AMPKα1(Ab-496) Antibody #21130.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPKα1(Ab-496) Antibody #21130(left) or the same antibody preincubated with blocking peptide(right).
背景

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Kim JE, et al. (2005) J Proteome Res. 4(4): 1339-1346.
Woods A, et al. (2003) J Biol Chem. 278(31): 28434-28442.

如果您使用该产品21130发表了文章,请通知我们,让我们可以引用您的文献。

et al,Construction of adiponectin-encoding plasmid DNA and overexpression in mice in vivo.In Gene on 2012 Jul 10 by Huang YN, Qi JH, et al..PMID:22561699, , (2012),
PMID: 22561699
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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