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YWHAQ Antibody#31247

YWHAQ Antibody
YWHAQ Antibody
是否有货: 有货
产品总价:
产品详情

产品名称YWHAQ Antibody

来源种属Rabbit

克隆性Polyclonal

应用ELISA WB IHC

种属反应性Hu Ms

特异性The antibody detects endogenous level of total YWHAQ protein.

免疫原类型Peptide-KLH

免疫原描述Synthetic peptide corresponding to a region derived from 140-154 amino acids of Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide

基因/蛋白名称YWHAQ

别名tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide, 1C5, HS1, 14-3-3

数据库入口号Genbank No.: NP_006817

UniprotNP_006817

浓度0.3mg/ml

配方Supplied at 0.6mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Predicted MW: 28kd
ELISA: 1:1000-1:5000
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:25-1:100

Gel: 10%+12% SDS-PAGE
Lane1:Mouse brain tissue lysate
Lane2: A549 cell lysate
Lane3:Human lymphoma tissue lysate
Lane4: NIH/3T3 cell lysate
Lane5:Human colon cancer tissue lysate
Lysates: 40 µg per lane
Primary antibody: 1/300 dilution
Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Exposure time: 5 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 31247 (YWHAQ Antibody) at dilution 1/20, on the right is treated with the synthetic peptide.
背景

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.?

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

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400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com