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PCNA Antibody#31002

PCNA Antibody
PCNA Antibody
是否有货: 有货
产品总价:
产品详情

产品名称PCNA Antibody

来源种属Rabbit

克隆性Polyclonal

应用ELISA WB IHC

种属反应性Hu

特异性The antibody detects endogenous level of total PCNA protein.

免疫原类型Recombinant Protein

免疫原描述Fusion protein corresponding to a region derived from 9-252 amino acids of Human Proliferating cell nuclear antigen

基因/蛋白名称PCNA

别名Proliferating cell nuclear antigen

应用详情

Predicted MW: 29kd
ELISA: 1:500-1:5000
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:25-1:100

Gel: 10%SDS-PAGE
Lysate: 40 µg Hela cell lysate
Primary antibody: 1/500 dilution
Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution
Exposure time: 1 minute
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 31002 (PCNA Antibody) at dilution 1/25, on the right is treated with the fusion protein.
背景

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

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Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com