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位置: 首页 > 磷酸化抗体 > MITF (Phospho-Ser180/73) Antibody

MITF (Phospho-Ser180/73) Antibody#12018

MITF (Phospho-Ser180/73) Antibody
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产品总价:
产品详情

产品名称MITF (Phospho-Ser180/73) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of MITF only when phosphorylated at Serine 180/73.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 180(P-N-S(p)-P-M)/Serine 73(Q-E-R(p)-R-E) derived from Human MITF.

基因/蛋白名称MITF

修饰Phospho

别名MI, WS2, CMM8, WS2A, bHLHe32

数据库入口号Swiss-Prot#: O75030;
NCBI Gene#: 4286;
NCBI Protein#: XP_005264811.1

UniprotO75030

实际分子量52kd

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Predicted MW: 52kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Mouse brain tissue using MITF (Phospho-Ser180/73) Antibody #12018.The lane on the right is treated with the antigen-specific peptide.
背景
Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

如果您使用该产品12018发表了文章,请通知我们,让我们可以引用您的文献。

et al,Dioscin Derived From Solanum Melongena L. "Usukawamarunasu" Attenuates 伪-MSH-Induced Melanogenesis in B16 Murine Melanoma Cells via Downregulation of Phospho-CREB and MITF .In J Food Sci on 2015 Oct by Atsuyoshi Nishina, Kodai Ebina et al..PMID:26352003, , (2015),
PMID: 26352003
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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