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位置: 首页 > 磷酸化抗体 > p70 S6 Kinase (Phospho-Thr229) Antibody

p70 S6 Kinase (Phospho-Thr229) Antibody#12021

p70 S6 Kinase (Phospho-Thr229) Antibody
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产品总价:
产品详情

产品名称p70 S6 Kinase (Phospho-Thr229) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at Threonine 229.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Threonine 229 (N-H-Q(p)-G-H) derived from Human p70 S6 Kinase.

基因/蛋白名称p70 S6 Kinase

修饰Phospho

别名S6K, PS6K, S6K1, STK14A, p70-S6K

数据库入口号Swiss-Prot#: P23443;
NCBI Gene#: 6198;
NCBI Protein#: XP_005257622.1

UniprotP23443

实际分子量70kd

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Predicted MW: 70kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from JK cells using p70 S6 Kinase (Phospho-Thr229) Antibody #12021.The lane on the right is treated with the antigen-specific peptide.
背景
Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the preinitiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at 'Thr-412', which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin.

如果您使用该产品12021发表了文章,请通知我们,让我们可以引用您的文献。

et al,Tuberous Sclerosis Complex Inactivation Disrupts Melanogenesis via mTORC1 Activation.In J Clin Invest on 2017 Jan 3 by Juxiang Cao, Magdalena E Tyburczy,et al..PMID:27918305, , (2017),
PMID: 27918305
et al,GAS 6-expressing and self-sustaining cancer cells in 3D spheroids activate the PDK-RSK-mTOR pathway for survival and drug resistance. In Mol Oncol on 2017 Oct by Christine Baumann, Axel Ullrich, et al..PMID: 28675785, , (2017),
PMID: 28675785
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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