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位置: 首页 > 磷酸化抗体 > TK (Phospho-Ser13) Antibody

TK (Phospho-Ser13) Antibody#12027

TK (Phospho-Ser13) Antibody
TK (Phospho-Ser13) Antibody
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产品总价:
产品详情

产品名称TK (Phospho-Ser13) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous level of TK only when phosphorylated at Serine 13.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 13(P-G-S(p)-P-S) derived from Human TK.

基因/蛋白名称TK

修饰Phospho

别名Thymidine kinase, cytosolic; TK1

数据库入口号Swiss-Prot#: P04183;
NCBI Gene#: 7083;
NCBI Protein#: XP_005257688.1

UniprotP04183

实际分子量25kd

浓度1.0mg/ml

配方Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:2000
Immunohistochemistry: 1:100~1:300

Western blot analysis of lysates from HeLa cells treated with paclitaxel 1uM 24h, using TK (Phospho-Ser13) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using TK (Phospho-Ser13) Antibody. The picture on the right is blocked with the phospho peptide.
背景

TK is a cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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025-58868422

Email Address

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