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位置: 首页 > 磷酸化抗体 > PTP1B (Phospho-Ser50) Antibody

PTP1B (Phospho-Ser50) Antibody#12039

PTP1B (Phospho-Ser50) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称PTP1B (Phospho-Ser50) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt Mk

特异性The antibody detects endogenous level of PTP1B only when phosphorylated at Serine 50.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 50(D-V-S(p)-P-F) derived from Human PTP1B.

基因/蛋白名称PTP1B

修饰Phospho

别名PTP1B

数据库入口号Swiss-Prot#: P18031;
NCBI Gene#: 5770;
NCBI Protein#: NP_001265547.1

UniprotP18031

实际分子量49kd

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Predicted MW: 49kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from COS-7 cells untreated or treated with UV using PTP1B (Phospho-Ser50) Antibody #12039.
背景

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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