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位置: 首页 > 磷酸化抗体 > p27 Kip1 (Phospho-Thr198) Antibody

p27 Kip1 (Phospho-Thr198) Antibody#12047

p27 Kip1 (Phospho-Thr198) Antibody
p27 Kip1 (Phospho-Thr198) Antibody
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产品总价:
产品详情

产品名称p27 Kip1 (Phospho-Thr198) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous level of p27 Kip1 only when phosphorylated at Threonine 198.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Threonine 198 (R-R-R(p)-Q-T) derived from Human p27 Kip1.

基因/蛋白名称p27 Kip1

修饰Phospho

别名KIP1, MEN4, CDKN4, MEN1B, P27KIP1

数据库入口号Swiss-Prot#: P46527;
NCBI Gene#: 1027;
NCBI Protein#: NP_004055.1

UniprotP46527

实际分子量27kd

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Predicted MW: 27kd
Western blotting: 1:500~1:1000

Western Blot analysis of VEC cells using Phospho-p27 (T198) Polyclonal Antibody
Western blot analysis of lysates from HeLa cells, HepG2 cells and COLO cells, using p27 Kip1 (Phospho-Thr198) Antibody. The lane on the right is blocked with the phospho peptide.
背景

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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