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位置: 首页 > 磷酸化抗体 > I kappaB- alpha (Phospho-Tyr305) Antibody

I kappaB- alpha (Phospho-Tyr305) Antibody#12050

I kappaB- alpha (Phospho-Tyr305) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称I kappaB- alpha (Phospho-Tyr305) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt Mk

特异性The antibody detects endogenous level of I kappaB- alpha only when phosphorylated at Tyrosine 305.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Tyrosine 305(L-P-Y(p)-D-D) derived from Human I kappaB- alpha.

基因/蛋白名称I kappaB- alpha

修饰Phospho

别名IKBA, MAD-3, NFKBI

数据库入口号Swiss-Prot#: P25963;
NCBI Gene#: 4792;
NCBI Protein#: NP_065390.1

UniprotP25963

实际分子量39kd

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Predicted MW: 39kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from COS-7 cells using I kappaB- alpha (Phospho-Tyr305) Antibody #12050.The lane on the left is treated with the antigen-specific peptide.
背景

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com