免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 磷酸化抗体 > Catenin- beta (Phospho-Tyr489) Antibody

Catenin- beta (Phospho-Tyr489) Antibody#12061

Catenin- beta (Phospho-Tyr489) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Catenin- beta (Phospho-Tyr489) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Catenin- beta only when phosphorylated at Tyrosine 489.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Tyrosine 489 (L-H-Y(p)-G-L) derived from Human Catenin- beta.

基因/蛋白名称Catenin- beta

修饰Phospho

别名CTNNB, MRD19, armadillo

数据库入口号Swiss-Prot#: P35222;
NCBI Gene#: 1499;
NCBI Protein#: NP_001091679.1

UniprotP35222

实际分子量92kd

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Predicted MW: 92kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Mouse renin using Catenin- beta (Phospho-Tyr489) Antibody #12061.The lane on the left is treated with the antigen-specific peptide.
背景
Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML.

如果您使用该产品12061发表了文章,请通知我们,让我们可以引用您的文献。

et al,AMD3100 inhibits the migration and differentiation of neural stem cells after spinal cord injury.In Sci Rep on 2017 Mar 6 by Jia-Ming Liu , Kai Zhao,et al..PMID: 28246405, , (2017),
PMID: 28246405
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com