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位置: 首页 > 磷酸化抗体 > AMPK β1(Phospho-Ser181) Antibody

AMPK β1(Phospho-Ser181) Antibody#12071

AMPK β1(Phospho-Ser181) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称AMPK β1(Phospho-Ser181) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of AMPK β1 only when phosphorylated at Serine 181.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 181 (L-S-S(p)-S-P) derived from Human AMPK β1.

基因/蛋白名称AMPK β1

修饰Phospho

别名AMPK, HAMPKb

数据库入口号Swiss-Prot#: Q9Y478;
NCBI Gene#: 5564;
NCBI Protein#: NP_006244.2

UniprotQ9Y478

实际分子量38kd

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Predicted MW: 38kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Rat kindey using AMPK β1(Phospho-Ser181) Antibody #12071.The lane on the right is treated with the antigen-specific peptide.
背景
Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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