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位置: 首页 > 抗体 > PAK1(Ab-212) Antibody

PAK1(Ab-212) Antibody#21160

PAK1(Ab-212) Antibody
PAK1(Ab-212) Antibody
PAK1(Ab-212) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称PAK1(Ab-212) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of total PAK1protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa. 210~214 (P-V-T-P-T) derived from Human PAK1.

基因/蛋白名称PAK1

别名p21-activated kinase 1

数据库入口号Swiss-Prot: Q13153
NCBI Protein: NP_001122092.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 68kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 and 3T3 cells using PAK1(Ab-212) Antibody #21160.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1(Ab-212) Antibody #21160(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using PAK1(Ab-212) Antibody #21160.
背景
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Alexander K, et al. (2004) Mol Cell Biol; 24: 2808-2819
Thiel DA, et al. (2002) Curr Biol; 12:1227-1232
Rashid T, et al. (2001) J. Biol. Chem; 276: 49043 - 49052.

如果您使用该产品21160发表了文章,请通知我们,让我们可以引用您的文献。

et al,PAK1 Is Involved in the Spindle Assembly during the First Meiotic Division in Porcine OocytesInInt J Mol SciOn2023 Jan 6byLei Peng?1,?Yijing He et al..PMID: 36674642, , (2023),
PMID: 36674642
et al,Uptake of Shiga鈥恡oxigenic Escherichia coli鈥匰ubAB by HeLa cells requires an actin锟?and lipid raft鈥恉ependent pathway.In Cell Microbiol on 2014 Oct by Sayaka Nagasawa 1 , Kohei Ogura et al..PMID:24844382, , (2014),
PMID: 24844382
Bingyuan Wang, Wei Ma, Xiaoling Xu et al,Phosphorylation of histone H3 on Ser10 by auto-phosphorylated PAK1 is not essential for chromatin condensation and meiotic progression in porcine oocytes, Journal of Animal Science and Biotechnology, 4(1):13 (2013),
PMID: 23521812
et al, PAK1 Regulates Spindle Microtubule Organization During Oocyte Meiotic Maturation.In Front Biosci (Elite Ed) on 2010 Jun 1 by Sheng-Li Lin , Shu-Tao Qi,et al..PMID: 20515799, , (2010),
PMID: 20515799
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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