HP1-gamma Monoclonal Antibody - SAB

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HP1-gamma Monoclonal Antibody#27171

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产品名称HP1-gamma Monoclonal Antibody

来源种属Mouse

克隆性Monoclonal

克隆号5G10-F7-A12

亚型IgG1

纯化Affinity purified

应用WB ICC IP IHC

种属反应性Ms Hu Mk Hm Rt

特异性This antibody detects endogenous levels of HP1-gamma and does not cross-react with related proteins.

免疫原类型Recombinant Protein

免疫原描述Purified recombinant human HP1-gamma protein fragments expressed in E.coli.

基因/蛋白名称HP1-gamma

别名CBX 3; CBX3; CBX3_HUMAN; Chromobox homolog 3 (HP1 gamma homolog, Drosophila); Chromobox homolog 3; Chromobox protein homolog 3; GAMMA; HECH; Heterochromatin like protein 1; Heterochromatin protein 1 homolog gamma; Heterochromatin protein HP1 gamma

数据库入口号Uniprot: Q13185

Gene ID: 11335

UniprotQ13185

实际分子量22kd

配方Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

保存store at -20˚C

应用详情

Western blotting: 1:1000

Immunocytochemistry: 1:200

Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-HP1-gamma antibody (dilution 1:200).

Western blot detection of HP1-gamma in Hela,3T3,C6,COS7 and CHO-K1 cell lysates using HP1-gamma antibody (1:1000 diluted).Predicted band size:22KDa.Observed band size:22KDa.

Immunoprecipitation analysis of Hela cell lysates using HP1-gamma antibody.

Immunohistochemical analysis of paraffin-embedded Prostate Cancer using HP1-gamma Mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

背景

Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

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应用

WB免疫印迹
IHC免疫组化
IF免疫荧光
ICC免疫细胞化学
FC流式细胞
IP免疫沉淀
E酶联免疫吸附法
DB免疫斑点法
ChIP染色质免疫沉淀
GICA胶体金免疫层析法
NC阴性对照

种属反应性

Hu人
Ms小鼠
Rt大鼠
Dm果蝇
C线虫
Mk猴
Rb兔
B牛
D狗
P猪
Hm仓鼠
ChHm中国仓鼠
Chk鸡

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