BRAP Monoclonal Antibody - SAB | Signalway Antibody

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BRAP Monoclonal Antibody#27218

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产品名称BRAP Monoclonal Antibody

来源种属Mouse

克隆性Monoclonal

克隆号1E7-C9-D10-B10

亚型IgG1

纯化Affinity purified

应用WB

种属反应性Hu

特异性This antibody detects endogenous levels of BRAP and does not cross-react with related proteins.

免疫原类型Recombinant Protein

免疫原描述Purified recombinant human BRAP protein fragments expressed in E.coli.

基因/蛋白名称BRAP

别名3010002G07Rik; BRAP2; BRCA1 associated protein; EC 6.3.2.; Galectin 2 binding protein; IMP; Impedes mitogenic signal propagation; Renal carcinoma antigen NY REN 63; RING finger protein 52; RNF52; zgc:92894.

数据库入口号Uniprot: Q7Z569

Gene ID: 8315

UniprotQ7Z569

实际分子量67kd

配方Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

保存store at -20˚C

应用详情

Western blotting: 1:1000

Western blot detection of BRAP in A549 and HUVEC cell lysates using BRAP antibody (1:500 diluted).Predicted band size:67KDa.Observed band size:67KDa.

Western blot analysis of extracts from CHO-K1 cells, transfected with a human pFLAG-CMV2-BRAP construct (A) or transfected with a human pFLAG-CMV2 construct (B), using BRAP antibody (1:1000 diluted).

背景

Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.

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应用

WB免疫印迹
IHC免疫组化
IF免疫荧光
ICC免疫细胞化学
FC流式细胞
IP免疫沉淀
E酶联免疫吸附法
DB免疫斑点法
ChIP染色质免疫沉淀
GICA胶体金免疫层析法
NC阴性对照

种属反应性

Hu人
Ms小鼠
Rt大鼠
Dm果蝇
C线虫
Mk猴
Rb兔
B牛
D狗
P猪
Hm仓鼠
ChHm中国仓鼠
Chk鸡

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