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位置: 首页 > 磷酸化抗体 > PKCB (Phospho-Ser661) Antibody

PKCB (Phospho-Ser661) Antibody#11905

PKCB (Phospho-Ser661) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称PKCB (Phospho-Ser661) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of PKCB only when phosphorylated at serine 661.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 661 (G-F-S(p)-Y-T) derived from Human PKCB.

基因/蛋白名称PKCB

修饰Phospho

别名PIM-1; EC 2.7.11.1;

数据库入口号Swiss-Prot#: P05771;
NCBI Gene#: 5579;
NCBI Protein#: NP_002729.2

UniprotP05771

实际分子量77kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from HeLa cells treated with Heatshock using Phospho-PKCB (Ser661) antibody #11905.The lane on the right is treated with the antigen-specific peptide.
背景

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1.

Zhu M, Chen J, Jiang H, Miao C (2013) Cardiovasc Diabetol 12, 13
Leonard TA, et al. (2011) Cell 144, 55-66
Qiao M, et al. (2010) Mol Cell 38, 512-23

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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