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位置: 首页 > 磷酸化抗体 > Ezrin (Phospho-Tyr478) Antibody

Ezrin (Phospho-Tyr478) Antibody#11908

Ezrin (Phospho-Tyr478) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Ezrin (Phospho-Tyr478) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 478.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 478 (P-V-Y(p)-E-P) derived from Human Ezrin.

基因/蛋白名称Ezrin

修饰Phospho

别名Cytovillin; EZRI; p81; VIL2; Villin 2

数据库入口号Swiss-Prot#: P15311;
NCBI Gene#: 7430;
NCBI Protein#: NP_001104547.1

UniprotP15311

实际分子量70kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from K562 cells treated with Na3VO4 using Phospho-Ezrin (Tyr478) antibody #11908.The lane on the right is treated with the antigen-specific peptide.
背景

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Murchie R, et al. (2014) Proc Natl Acad Sci U S A 111, 693-8 . Mak H, et al. (2012)BMC Cancer 12, 82. Heiska L, et al. (2011)Oncogene 30, 4953-62 .

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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