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位置: 首页 > 磷酸化抗体 > CDK5 (Phospho-Tyr15) Antibody

CDK5 (Phospho-Tyr15) Antibody#11918

CDK5 (Phospho-Tyr15) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称CDK5 (Phospho-Tyr15) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of CDK5 only when phosphorylated at tyrosine 15.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Tyrosine15 (G-T-Y(p)-G-T) derived from Human CDK5.

基因/蛋白名称CDK5

修饰Phospho

别名CDK5; CRK6; Cell division protein kinase 5; Tau protein kinase II catalytic subunit; kinase Cdk5

数据库入口号Swiss-Prot#: Q00535;
NCBI Gene#: 1020;
NCBI Protein#: NP_001157882.1

UniprotQ00535

实际分子量33kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from COS7 cells treated with EGF using Phospho-CDK5 (Tyr15) antibody #11918.The lane on the right is treated with the antigen-specific peptide.
背景

Proline-directed serine/threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. Interacts with D1 and D3-type G1 cyclins. Phosphorylates SRC, NOS3, VIM/vimentin, p35/CDK5R1, MEF2A, SIPA1L1, SH3GLB1, PXN, PAK1, MCAM/MUC18, SEPT5, SYN1, DNM1, AMPH, SYNJ1, CDK16, RAC1, RHOA, CDC42, TONEBP/NFAT5, MAPT/TAU, MAP1B, histone H1, p53/TP53, HDAC1, APEX1, PTK2/FAK1, huntingtin/HTT, ATM, MAP2, NEFH and NEFM.

Miao Y, et al. (2012)PLoS One 7, e42318. Li P, et al. (2011)Cell 147, 815-26. Nohara A, et al. (2011)J Biol Chem 286, 33457-65.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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