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位置: 首页 > 磷酸化抗体 > Smad2 (Phospho-Ser250) Antibody

Smad2 (Phospho-Ser250) Antibody#11924

Smad2 (Phospho-Ser250) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Smad2 (Phospho-Ser250) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Smad2 only when phosphorylated at serine 250.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine250(E-L-S(p)-P-T) derived from Human Smad2 .

基因/蛋白名称Smad2

修饰Phospho

别名JV18-1; MADH2; Mad-related protein 2; Mothers against DPP homolog 2;

数据库入口号Swiss-Prot#: Q15796;
NCBI Gene#: 4087;
NCBI Protein#: NP_001003652.1

UniprotQ15796

实际分子量65kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from 293 cells treated with PMA using Phospho-Smad2 (Ser250) antibody #11924.The lane on the right is treated with the antigen-specific peptide.
背景

Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Fransvea E, Mazzocca A, Antonaci S, Giannelli G (2009)Hepatology 49, 839-50 . Funaba M, Zimmerman CM, Mathews LS (2002)J Biol Chem 277, 41361-8. Kretzschmar M, Doody J, Timokhina I, Massagu¨¦ J (1999)Genes Dev 13, 804-16 .

如果您使用该产品11924发表了文章,请通知我们,让我们可以引用您的文献。

et al,TGF β1 mediated Smad signaling pathway and EMT in hepatic fibrosis induced by Nano NiO in vivo and in vitro.In Environ Toxicol on 2020 Apr by Zhang Q, Chang X ,et al..PMID: 31737983, , (2020),
PMID: 31737983
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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