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位置: 首页 > 磷酸化抗体 > IKK-γ (Phospho-Ser85) Antibody

IKK-γ (Phospho-Ser85) Antibody#11927

IKK-γ (Phospho-Ser85) Antibody
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产品总价:
产品详情

产品名称IKK-γ (Phospho-Ser85) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms

特异性The antibody detects endogenous level of IKK-γ only when phosphorylated at serine 85.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 85 (Q-A-S(p)-Q-R) derived from Human IKK-gamma.

基因/蛋白名称IKK-γ

修饰Phospho

别名FIP-3; FIP3; I-kappa-B kinase gamma; IKK-gamma; IKKAP1

数据库入口号Swiss-Prot#: Q9Y6K9;
NCBI Gene#: 8517;
NCBI Protein#: NP_001093326.2

UniprotQ9Y6K9

实际分子量48kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HepG2 cells treated with Anisomycin using Phospho-IKK-gamma (Ser85) antibody #11927.The lane on the right is treated with the antigen-specific peptide.
背景

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'-linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.

Niu J, Shi Y, Iwai K, Wu ZH (2011) EMBO J 30, 3741-53. Wu ZH, et al. (2010)Mol Cell 40, 75-86. Palkowitsch L, Leidner J, Ghosh S, Marienfeld RB (2008)J Biol Chem 283, 76-86.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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