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位置: 首页 > 磷酸化抗体 > IKK- alpha/ beta (Phospho-Ser180/181) Antibody

IKK- alpha/ beta (Phospho-Ser180/181) Antibody#11930

IKK- alpha/ beta (Phospho-Ser180/181) Antibody
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产品总价:
产品详情

产品名称IKK- alpha/ beta (Phospho-Ser180/181) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of IKK- alpha/beta only when phosphorylated at serine 180/181.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 180/181 (C-T-S(p)-F-V) derived from Human IKK- alpha/ beta.

基因/蛋白名称IKK- alpha/ beta

修饰Phospho

别名FLJ40509; I-kappa-B kinase; IKBKB; kinase beta; NFKBIKB

数据库入口号Swiss-Prot#: O15111/O14920;
NCBI Gene#: 1147;
NCBI Protein#: NP_001269.3

UniprotO15111/O14920

实际分子量85kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HepG2 cells treated with TNF using IKK- alpha/ beta (Phospho-Ser180/181) antibody #11930.The lane on the right is treated with the antigen-specific peptide.
背景

Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.

Hafeez BB, et al. (2008) Cancer Res 68, 8564-72
Cahill CM, Rogers JT (2008) J Biol Chem 283, 25900-12
Encinas M, et al. (2008)Cell Death Differ 15, 1510-21

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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