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位置: 首页 > 磷酸化抗体 > GR (Phospho-Ser226) Antibody

GR (Phospho-Ser226) Antibody#11937

GR (Phospho-Ser226) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称GR (Phospho-Ser226) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous level of GR only when phosphorylated at serine 226.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 226 (L-L-S(p)-P-L) derived from Human GR.

基因/蛋白名称GR

修饰Phospho

别名GCCR; GCR; GRL; NR3C1;

数据库入口号Swiss-Prot#: P04150;
NCBI Gene#: 2908;
NCBI Protein#: NP_000167.1

UniprotP04150

实际分子量86kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Jurkat cells treated with EGF using Phospho-GR (Ser226) antibody #11937.The lane on the right is treated with the antigen-specific peptide.
背景

Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation.

Wehmeyer L, Du Toit A, Lang DM, Hapgood JP (2014) J Biol Chem 289, 10235-51
Bouazza B, et al. (2014) Am J Respir Cell Mol Biol 50, 301-15
Lee MS, et al. (2013) Behav Brain Res 236, 56-61

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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