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位置: 首页 > 磷酸化抗体 > CDC25B (Phospho-Ser353) Antibody

CDC25B (Phospho-Ser353) Antibody#11949

CDC25B (Phospho-Ser353) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称CDC25B (Phospho-Ser353) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms

特异性The antibody detects endogenous level of CDC25B only when phosphorylated at serine 353.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 353(R-R-S(p)-V-T) derived from Human CDC25B .

基因/蛋白名称CDC25B

修饰Phospho

别名CDC25M2; MPIP2; Dual specificity phosphatase Cdc25B; CDC25HU2;

数据库入口号Swiss-Prot#: P30305;
NCBI Gene#: 994;
NCBI Protein#: NP_001274445.1

UniprotP30305

实际分子量64kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from COS7 cells treated with Etoposide using Phospho-CDC25B (Ser353) antibody #11949.The lane on the right is treated with the antigen-specific peptide.
背景

Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

Reboutier D, et al. (2012) J Cell Biol 197, 19-26
Lobjois V, et al. (2011) Biochem Biophys Res Commun 410, 87-90
Feng C, et al. (2007) Biol Reprod 77, 560-8

如果您使用该产品11949发表了文章,请通知我们,让我们可以引用您的文献。

et al,Aurora kinase A is essential for meiosis in mouse oocytes. In PLoS Genet on 2021 Apr 26 by Cecilia S Blengini, Patricia Ibrahimian,et al..PMID:33901174, , (2021),
PMID: 33901174
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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