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位置: 首页 > 磷酸化抗体 > ETK (Phospho-Tyr566) Antibody

ETK (Phospho-Tyr566) Antibody#11953

ETK (Phospho-Tyr566) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称ETK (Phospho-Tyr566) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms

特异性The antibody detects endogenous level of ETK only when phosphorylated at tyrosine 566.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Tyrosine566 (D-Q-Y(p)-V-S) derived from Human ETK.

基因/蛋白名称ETK

修饰Phospho

别名BMX; BMX NON-receptor tyrosine kinase; Bone marrow kinase BMX; Epithelial and endothelial tyrosine kinase; NTK38

数据库入口号Swiss-Prot#: P51813;
NCBI Gene#: 660;
NCBI Protein#: NP_001712.1

UniprotP51813

实际分子量78kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HeLa cells treated with Serum using Phospho-ETK (Tyr566) antibody #11953.The lane on the right is treated with the antigen-specific peptide.
背景

Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility.

Zhang R, et al. (2003) J Biol Chem 278, 51267-76
Tsai YT, et al. (2000) Mol Cell Biol 20, 2043-54

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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