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位置: 首页 > 磷酸化抗体 > Smad3 (Phospho-Thr179) Antibody

Smad3 (Phospho-Thr179) Antibody#11955

Smad3 (Phospho-Thr179) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Smad3 (Phospho-Thr179) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Smad3 only when phosphorylated at threonine 179.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine179(P-E-T(p)-P-P) derived from Human Smad3 .

基因/蛋白名称Smad3

修饰Phospho

别名JV15-2; MAD-3; MADH3; Smad 3; Mothers against decapentaplegic homolog 3

数据库入口号Swiss-Prot#: P84022;
NCBI Gene#: 4088;
NCBI Protein#: NP_001138574.1

UniprotP84022

实际分子量50kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HeLa cells treated with TNF using Phospho-Smad3 (Thr179) antibody #11955.The lane on the right is treated with the antigen-specific peptide.
背景

Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Cohen-Solal KA, et al. (2011) Pigment Cell Melanoma Res 24, 512-24
Zelivianski S, Cooley A, Kall R, Jeruss JS (2010) Mol
Cancer Res 8, 1375-87
Matsuura I, et al. (2010) J Biol Chem 285, 1754-64

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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