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位置: 首页 > 磷酸化抗体 > XIAP (Phospho-Ser87) Antibody

XIAP (Phospho-Ser87) Antibody#11956

XIAP (Phospho-Ser87) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称XIAP (Phospho-Ser87) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous level of XIAP only when phosphorylated at serine 87.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine87(K-V-S(p)-P-N) derived from Human XIAP .

基因/蛋白名称XIAP

修饰Phospho

别名API3; BIR4; BIRC4; IAP3; X-linked IAP

数据库入口号Swiss-Prot#: P98170;
NCBI Gene#: 331;
NCBI Protein#: NP_001158.2

UniprotP98170

实际分子量57kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HepG2 cells treated with Anisomycin using Phospho-XIAP (Ser87) antibody #11956.The lane on the right is treated with the antigen-specific peptide.
背景

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, copper homeostasis, mitogenic kinase signaling, cell proliferation, as well as cell invasion and metastasis. Acts as a direct caspase inhibitor. Directly bind to the active site pocket of CASP3 and CASP7 and obstructs substrate entry. Inactivates CASP9 by keeping it in a monomeric, inactive state.

Huang X, Wu Z, Mei Y, Wu M (2013)EMBO J 32, 2204-16. Bornhauser BC, et al. (2007) Blood 110, 2084-91.Dan HC, et al. (2004)J Biol Chem 279, 5405-12.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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