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位置: 首页 > 磷酸化抗体 > Pin1 (Phospho-Ser16) Antibody

Pin1 (Phospho-Ser16) Antibody#11960

Pin1 (Phospho-Ser16) Antibody
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产品总价:
产品详情

产品名称Pin1 (Phospho-Ser16) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms

特异性The antibody detects endogenous level of Pin1 only when phosphorylated at serine 16.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine16(R-M-S(p)-R-S) derived from Human Pin1 .

基因/蛋白名称Pin1

修饰Phospho

别名PPIase Pin1; Rotamase Pin1; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; EC 5.2.1.8;

数据库入口号Swiss-Prot#: Q13526;
NCBI Gene#: 5300;
NCBI Protein#: NP_006212.1

UniprotQ13526

实际分子量18kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from COS7 cells treated with Insulin using Phospho-Pin1 (Ser16) antibody #11960.The lane on the right is treated with the antigen-specific peptide.
背景

Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzes pSer/Thr-Pro cis/trans isomerizations. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation. Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner.

Lee YC, et al. (2013)J Cell Sci 126, 4862-72. Ando K, et al. (2013)Neurobiol Aging 34, 757-69. Nechama M, Lin CL, Richter JD (2013)Mol Cell Biol 33, 48-58.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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