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位置: 首页 > 磷酸化抗体 > c-Met (Phospho-Tyr1003) Antibody

c-Met (Phospho-Tyr1003) Antibody#11971

c-Met (Phospho-Tyr1003) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称c-Met (Phospho-Tyr1003) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of c-Met only when phosphorylated at tyrosine 1003.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 1003 (V-D-Y(p)-R-A) derived from Human c-Met.

基因/蛋白名称c-Met

修饰Phospho

别名HGF receptor; HGF-SF receptor; Hepatocyte growth factor receptor precursor; Hepatocyte growth factor receptor precursor; Met proto- oncogene tyros

数据库入口号Swiss-Prot#: P08581;
NCBI Gene#: 4233;
NCBI Protein#: NP_000236.2

UniprotP08581

实际分子量155kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HepG2 tissue using c-Met (Phospho-Tyr1003) antibody #11971.The lane on the right is treated with the antigen-specific peptide.
背景

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.

Usatyuk PV, et al. (2014)J Biol Chem. Asiedu MK, et al. (2014) Oncogene 33, 1316-24. Dulak AM, et al. (2011) Oncogene 30, 3625-35.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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