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位置: 首页 > 磷酸化抗体 > Cyclin D1 (Phospho-Ser90) Antibody

Cyclin D1 (Phospho-Ser90) Antibody#11986

Cyclin D1 (Phospho-Ser90) Antibody
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产品总价:
产品详情

产品名称Cyclin D1 (Phospho-Ser90) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous level of Cyclin D1 only when phosphorylated at serine 90.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 90(F-L-S(p)-L-E) derived from Human Cyclin D1 .

基因/蛋白名称Cyclin D1

修饰Phospho

别名BCL1; CCND1; CYL-1; PRAD1; PRAD1 oncogene

数据库入口号Swiss-Prot#: P24385;
NCBI Gene#: 595;
NCBI Protein#: NP_444284.1

UniprotP24385

实际分子量31kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western Blot analysis of lysates of MCF7 treated with LPS and MCF7, using primary antibody at 1:1000 dilution.
背景

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.

Sewing A, M¨¹ller R (1994) Oncogene 9, 2733-6

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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