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位置: 首页 > 磷酸化抗体 > Epo-R (Phospho-Tyr368) Antibody

Epo-R (Phospho-Tyr368) Antibody#11996

Epo-R (Phospho-Tyr368) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Epo-R (Phospho-Tyr368) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms

特异性The antibody detects endogenous level of Epo-R only when phosphorylated at tyrosine 368.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Tyrosine 368 (D-T-Y(p)-L-V) derived from Human Epo-R.

基因/蛋白名称Epo-R

修饰Phospho

别名EPO R; EPO-R; epor;

数据库入口号Swiss-Prot#: P19235;
NCBI Gene#: 2057;
NCBI Protein#: NP_000112.1

UniprotP19235

实际分子量55kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from K562 tissue using Epo-R (Phospho-Tyr368) antibody #11996.The lane on the right is treated with the antigen-specific peptide.
背景

Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase. Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.

Funakoshi-Tago M, et al. (2010) J Biol Chem 285, 5296-307
Sulahian R, Cleaver O, Huang LJ (2009) Blood 113, 5287-97
Zhang YL, et al. (2009) Mol Cell 33, 266-74

如果您使用该产品11996发表了文章,请通知我们,让我们可以引用您的文献。

5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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