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位置: 首页 > 磷酸化抗体 > IGFBP-3 (Phospho-Ser183) Antibody

IGFBP-3 (Phospho-Ser183) Antibody#11999

IGFBP-3 (Phospho-Ser183) Antibody
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产品总价:
产品详情

产品名称IGFBP-3 (Phospho-Ser183) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Rt

特异性The antibody detects endogenous level of IGFBP-3 only when phosphorylated at serine 183.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 183(K-D-S(p)-Q-R) derived from Human IGFBP-3 .

基因/蛋白名称IGFBP-3

修饰Phospho

别名IBP-3; IBP3; IGF-binding protein 3; IGFBP3; Insulin-like growth factor binding protein 3 precursor

数据库入口号Swiss-Prot#: P17936;
NCBI Gene#: 3486;
NCBI Protein#: NP_000589.2

UniprotP17936

实际分子量30kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Rat brain tissue using IGFBP-3 (Phospho-Ser183) antibody #11999.The lane on the right is treated with the antigen-specific peptide.
背景

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Zhang Q, Steinle JJ (2013)Invest Ophthalmol Vis Sci 54, 3052-7 . Cobb LJ, Liu B, Lee KW, Cohen P (2006) Cancer Res 66, 10878-84 .

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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025-58868422

Email Address

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