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位置: 首页 > 磷酸化抗体 > Amyloid β A4 (Phospho-Thr743/668) Antibody

Amyloid β A4 (Phospho-Thr743/668) Antibody#11643

Amyloid β A4 (Phospho-Thr743/668) Antibody
Amyloid β A4 (Phospho-Thr743/668) Antibody
Amyloid β A4 (Phospho-Thr743/668) Antibody
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产品总价:
产品详情

产品名称Amyloid β A4 (Phospho-Thr743/668) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of Amyloid β A4 only when phosphorylated at threonine 743/668.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine743 /668 (A-V-T(p)-P-E) derived from Human Amyloid β A4.

基因/蛋白名称Amyloid β A4

修饰Phospho

别名A4; AD1; APP; APPI; Beta-amyloid protein 42

数据库入口号Swiss-Prot#: P05067;
NCBI Gene#: 351;
NCBI Protein#: NP_000475.1.

UniprotP05067

实际分子量140kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from cos-7 cells (Lane 2), using Amyloid β A4 (Phospho-Thr743/668) Antibody #11643. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using Amyloid β A4 (phospho-Thr743/668) antibody #11643 (left)or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed HeLa cells using Amyloid β A4 (phospho-Thr743/668) Antibody #11643.
背景

Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity By similarity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1.

Ming-Sum Lee J. Cell Biol., Oct 2003; 163: 83.
Tadashi Nakaya and Toshiharu Suzuki Genes Cells, Jun 2006; 11: 633 - 645
Keun-A Chang, Mol. Cell. Biol., Jun 2006; 26: 4327 ¨C 4338.
Thor D. Stein J. Neurosci., Sep 2004; 24: 7707 - 7717

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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