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位置: 首页 > 磷酸化抗体 > Synaptotagmin (Phospho-Thr202) Antibody

Synaptotagmin (Phospho-Thr202) Antibody#11645

Synaptotagmin (Phospho-Thr202) Antibody
Synaptotagmin (Phospho-Thr202) Antibody
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产品总价:
产品详情

产品名称Synaptotagmin (Phospho-Thr202) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of Synaptotagmin only when phosphorylated at threonine 202.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 202(R-K-T(p)-L-N) derived from Human Synaptotagmin.

基因/蛋白名称Synaptotagmin

修饰Phospho

别名LAG; LAP18; STN1; Prosolin; stathmin

数据库入口号Swiss-Prot#: P21579;
NCBI Gene#: 6857;
NCBI Protein#: NP_001129277.1

UniprotP21579

实际分子量60kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from colo cells (Lane 2), using Synaptotagmin (Phospho-Thr202) Antibody #11645. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Synaptotagmin (phospho-Thr202) antibody #11645 (left)or the same antibody preincubated with blocking peptide (right).
背景

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse.

E Carafoli, Crit. Rev. Biochem. Mol. Biol., Apr 2001; 36: 107.
Gry Houeland, J Neurophysiol, Jan 2007; 97: 134 - 143.
Christelle Carteron, J. Cell Sci., Mar 2006; 119: 898 - 909.
Vladimir I. Slepnev, Science, Aug 1998; 281: 821.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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